LOCUS       pSK-BNT-Swt        7197 bp    DNA     circular SYN 14-FEB-2022
SOURCE      synthetic DNA construct.
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7197)
  AUTHORS   Demo User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jan 21, 2022 from SnapGene Viewer 5.1.7 to Vector 
            NTI(R) format
            https://www.snapgene.com
COMMENT     Storage Type: Basic
            Original Author:
            Demo User
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-14264406|
COMMENT     VNTDBDATE|-14264406|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pSK-BNT-Swt|
COMMENT     VNTAUTHORNAME|Demo User|
FEATURES             Location/Qualifiers
     misc_feature    complement(263..569)
                     /vntifkey="21"
                     /label=f1
     misc_feature    754..772
                     /vntifkey="21"
                     /label=T7\promoter
     misc_feature    771..823
                     /vntifkey="21"
                     /label=5'\\UTR
     misc_feature    773..5070
                     /vntifkey="21"
                     /label=Cassette\A
     primer          794..823
                     /vntifkey="27"
                     /label=PBNT5end
                     /note="sequence: TCCCCACAGACTCAGAGAGAACCCGCCACC"
     primer          complement(794..823)
                     /vntifkey="27"
                     /label=MBNT5end
                     /note="sequence: GGTGGCGGGTTCTCTCTGAGTCTGTGGGGA"
     CDS             824..4642
                     /codon_start=1
                     /vntifkey="4"
                     /label=S\protein
     primer          2060..2080
                     /vntifkey="27"
                     /label=PBNT1292
                     /note="sequence: ggacagacaggcaagatcgcc"
     primer          2132..2161
                     /vntifkey="27"
                     /label=PBNTlig1
                     /note="sequence: AACAGCAACAACCTGGACTCCAAAGTCGGC"
     primer          complement(2132..2161)
                     /vntifkey="27"
                     /label=MBNTlig1
                     /note="sequence: GCCGACTTTGGAGTCCAGGTTGTTGCTGTT"
     misc_feature    2177..2179
                     /vntifkey="21"
                     /label=L452
     misc_feature    2273..2275
                     /vntifkey="21"
                     /label=E484
     misc_feature    2324..2326
                     /vntifkey="21"
                     /label=N501
     misc_feature    2663..2664
                     /vntifkey="21"
                     /label=D614
     misc_feature    2864..2866
                     /vntifkey="21"
                     /label=P681
     primer          3461..3490
                     /vntifkey="27"
                     /label=PBNTlig2
                     /note="sequence: GGCACAATCACAAGCGGCTGGACATTTGGA"
     primer          complement(3461..3490)
                     /vntifkey="27"
                     /label=MBNTlig2
                     /note="sequence: TCCAAATGTCCAGCCGCTTGTGATTGTGCC"
     primer          3653..3691
                     /vntifkey="27"
                     /label=P-BNT950N
                     /note="sequence: gccctgggaaagctgcagaacgtggtcaaccagaatgcc"
     misc_feature    3671..3673
                     /vntifkey="21"
                     /label=D950
     primer          4139..4159
                     /vntifkey="27"
                     /label=PBNT3387
                     /note="sequence: cagcggaacttctacgagccc"
     primer          4652..4681
                     /vntifkey="27"
                     /label=PBNT3end
                     /note="sequence: GAGCTGGTACTGCATGCACGCAATGCTAGC"
     primer          complement(4652..4681)
                     /vntifkey="27"
                     /label=MBNT3end
                     /note="sequence: GCTAGCATTGCGTGCATGCAGTACCAGCTC"
     misc_feature    4655..4937
                     /vntifkey="21"
                     /label=3'\UTR
     misc_feature    4944..5054
                     /vntifkey="21"
                     /label=polyA
     primer          complement(5136..5158)
                     /vntifkey="27"
                     /label=T3P
                     /note="sequence: cgcaattaaccctcactaaaggg"
     misc_feature    complement(5136..5155)
                     /vntifkey="21"
                     /label=T3\P
     primer          complement(5460..5481)
                     /vntifkey="27"
                     /label=PT6265\seq
                     /note="sequence: cgtattaccgcctttgagtgag"
     primer          5514..5537
                     /vntifkey="27"
                     /label=P-DL-1
                     /note="sequence: agaacatgtgagcaaaaggccagc"
     misc_feature    complement(5522..6189)
                     /vntifkey="21"
                     /label=pUC\ori
     primer          complement(6193..6214)
                     /vntifkey="27"
                     /label=M-Pica-3561
                     /note="sequence: ttcgttccactgagcgtcagac"
     misc_feature    complement(6340..7197)
                     /vntifkey="21"
                     /label=Ampicillin
BASE COUNT     1843 a      2040 c      1799 g      1515 t 
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa 
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa 
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac 
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa 
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct 
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa 
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc 
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc 
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 
      721 cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg actcactata ggaataaact 
      781 agtattcttc tggtccccac agactcagag agaacccgcc accatgttcg tgttcctggt 
      841 gctgctgcct ctggtgtcca gccagtgtgt gaacctgacc accagaacac agctgcctcc 
      901 agcctacacc aacagcttta ccagaggcgt gtactacccc gacaaggtgt tcagatccag 
      961 cgtgctgcac tctacccagg acctgttcct gcctttcttc agcaacgtga cctggttcca 
     1021 cgccatccac gtgtccggca ccaatggcac caagagattc gacaaccccg tgctgccctt 
     1081 caacgacggg gtgtactttg ccagcaccga gaagtccaac atcatcagag gctggatctt 
     1141 cggcaccaca ctggacagca agacccagag cctgctgatc gtgaacaacg ccaccaacgt 
     1201 ggtcatcaaa gtgtgcgagt tccagttctg caacgacccc ttcctgggcg tctactacca 
     1261 caagaacaac aagagctgga tggaaagcga gttccgggtg tacagcagcg ccaacaactg 
     1321 caccttcgag tacgtgtccc agcctttcct gatggacctg gaaggcaagc agggcaactt 
     1381 caagaacctg cgcgagttcg tgtttaagaa catcgacggc tacttcaaga tctacagcaa 
     1441 gcacacccct atcaacctcg tgcgggatct gcctcagggc ttctctgctc tggaacccct 
     1501 ggtggatctg cccatcggca tcaacatcac ccggtttcag acactgctgg ccctgcacag 
     1561 aagctacctg acacctggcg atagcagcag cggatggaca gctggtgccg ccgcttacta 
     1621 tgtgggctac ctgcagccta gaaccttcct gctgaagtac aacgagaacg gcaccatcac 
     1681 cgacgccgtg gattgtgctc tggatcctct gagcgagaca aagtgcaccc tgaagtcctt 
     1741 caccgtggaa aagggcatct accagaccag caacttccgg gtgcagccca ccgaatccat 
     1801 cgtgcggttc cccaatatca ccaatctgtg ccccttcggc gaggtgttca atgccaccag 
     1861 attcgcctct gtgtacgcct ggaaccggaa gcggatcagc aattgcgtgg ccgactactc 
     1921 cgtgctgtac aactccgcca gcttcagcac cttcaagtgc tacggcgtgt cccctaccaa 
     1981 gctgaacgac ctgtgcttca caaacgtgta cgccgacagc ttcgtgatcc ggggagatga 
     2041 agtgcggcag attgcccctg gacagacagg caagatcgcc gactacaact acaagctgcc 
     2101 cgacgacttc accggctgtg tgattgcctg gaacagcaac aacctggact ccaaagtcgg 
     2161 cggcaactac aattacctgt accggctgtt ccggaagtcc aatctgaagc ccttcgagcg 
     2221 ggacatctcc accgagatct atcaggccgg cagcacccct tgtaacggcg tggaaggctt 
     2281 caactgctac ttcccactgc agtcctacgg ctttcagccc acaaatggcg tgggctatca 
     2341 gccctacaga gtggtggtgc tgagcttcga actgctgcat gcccctgcca cagtgtgcgg 
     2401 ccctaagaaa agcaccaatc tcgtgaagaa caaatgcgtg aacttcaact tcaacggcct 
     2461 gaccggcacc ggcgtgctga cagagagcaa caagaagttc ctgccattcc agcagtttgg 
     2521 ccgggatatc gccgatacca cagacgccgt tagagatccc cagacactgg aaatcctgga 
     2581 catcacccct tgcagcttcg gcggagtgtc tgtgatcacc cctggcacca acaccagcaa 
     2641 tcaggtggca gtgctgtacc aggacgtgaa ctgtaccgaa gtgcccgtgg ccattcacgc 
     2701 cgatcagctg acacctacat ggcgggtgta ctccaccggc agcaatgtgt ttcagaccag 
     2761 agccggctgt ctgatcggag ccgagcacgt gaacaatagc tacgagtgcg acatccccat 
     2821 cggcgctgga atctgcgcca gctaccagac acagacaaac agccctcgga gagccagaag 
     2881 cgtggccagc cagagcatca ttgcctacac aatgtctctg ggcgccgaga acagcgtggc 
     2941 ctactccaac aactctatcg ctatccccac caacttcacc atcagcgtga ccacagagat 
     3001 cctgcctgtg tccatgacca agaccagcgt ggactgcacc atgtacatct gcggcgattc 
     3061 caccgagtgc tccaacctgc tgctgcagta cggcagcttc tgcacccagc tgaatagagc 
     3121 cctgacaggg atcgccgtgg aacaggacaa gaacacccaa gaggtgttcg cccaagtgaa 
     3181 gcagatctac aagacccctc ctatcaagga cttcggcggc ttcaatttca gccagattct 
     3241 gcccgatcct agcaagccca gcaagcggag cttcatcgag gacctgctgt tcaacaaagt 
     3301 gacactggcc gacgccggct tcatcaagca gtatggcgat tgtctgggcg acattgccgc 
     3361 cagggatctg atttgcgccc agaagtttaa cggactgaca gtgctgcctc ctctgctgac 
     3421 cgatgagatg atcgcccagt acacatctgc cctgctggcc ggcacaatca caagcggctg 
     3481 gacatttgga gcaggcgccg ctctgcagat cccctttgct atgcagatgg cctaccggtt 
     3541 caacggcatc ggagtgaccc agaatgtgct gtacgagaac cagaagctga tcgccaacca 
     3601 gttcaacagc gccatcggca agatccagga cagcctgagc agcacagcaa gcgccctggg 
     3661 aaagctgcag gacgtggtca accagaatgc ccaggcactg aacaccctgg tcaagcagct 
     3721 gtcctccaac ttcggcgcca tcagctctgt gctgaacgat atcctgagca gactggaccc 
     3781 tcctgaggcc gaggtgcaga tcgacagact gatcacaggc agactgcaga gcctccagac 
     3841 atacgtgacc cagcagctga tcagagccgc cgagattaga gcctctgcca atctggccgc 
     3901 caccaagatg tctgagtgtg tgctgggcca gagcaagaga gtggactttt gcggcaaggg 
     3961 ctaccacctg atgagcttcc ctcagtctgc ccctcacggc gtggtgtttc tgcacgtgac 
     4021 atatgtgccc gctcaagaga agaatttcac caccgctcca gccatctgcc acgacggcaa 
     4081 agcccacttt cctagagaag gcgtgttcgt gtccaacggc acccattggt tcgtgacaca 
     4141 gcggaacttc tacgagcccc agatcatcac caccgacaac accttcgtgt ctggcaactg 
     4201 cgacgtcgtg atcggcattg tgaacaatac cgtgtacgac cctctgcagc ccgagctgga 
     4261 cagcttcaaa gaggaactgg acaagtactt taagaaccac acaagccccg acgtggacct 
     4321 gggcgatatc agcggaatca atgccagcgt cgtgaacatc cagaaagaga tcgaccggct 
     4381 gaacgaggtg gccaagaatc tgaacgagag cctgatcgac ctgcaagaac tggggaagta 
     4441 cgagcagtac atcaagtggc cctggtacat ctggctgggc tttatcgccg gactgattgc 
     4501 catcgtgatg gtcacaatca tgctgtgttg catgaccagc tgctgtagct gcctgaaggg 
     4561 ctgttgtagc tgtggcagct gctgcaagtt cgacgaggac gattctgagc ccgtgctgaa 
     4621 gggcgtgaaa ctgcactaca catgatgact cgagctggta ctgcatgcac gcaatgctag 
     4681 ctgccccttt cccgtcctgg gtaccccgag tctcccccga cctcgggtcc caggtatgct 
     4741 cccacctcca cctgccccac tcaccacctc tgctagttcc agacacctcc caagcacgca 
     4801 gcaatgcagc tcaaaacgct tagcctagcc acacccccac gggaaacagc agtgattaac 
     4861 ctttagcaat aaacgaaagt ttaactaagc tatactaacc ccagggttgg tcaatttcgt 
     4921 gccagccaca ccctggagct agcaaaaaaa aaaaaaaaaa aaaaaaaaaa aaagcatatg 
     4981 actaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 
     5041 aaaaaaaaaa aaaagcttga tatcgaattc ctgcagcccg ggggatccac tagttctaga 
     5101 gcggccgcca ccgcggtgga gctccagctt ttgttccctt tagtgagggt taattgcgcg 
     5161 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc 
     5221 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta 
     5281 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca 
     5341 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc 
     5401 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 
     5461 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 
     5521 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 
     5581 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 
     5641 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 
     5701 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 
     5761 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 
     5821 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 
     5881 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 
     5941 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 
     6001 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt 
     6061 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 
     6121 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 
     6181 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 
     6241 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 
     6301 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc 
     6361 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta 
     6421 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga 
     6481 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg 
     6541 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc 
     6601 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat 
     6661 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag 
     6721 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat 
     6781 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa 
     6841 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa 
     6901 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga 
     6961 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg 
     7021 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc 
     7081 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg 
     7141 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcat 
//
